· Hieff Clone® Plus Multi One Step Cloning Kit 10912ES（）Yeasen，Yeasen。（）Hieff Clone® Plus
· ClonExpress MultiS One Step Cloning Kit （）（C113） ￥520 TUNEL BrightGreen Apoptosis Detection Kit（）（A112） ￥1780
ClonExpress MultiS One Step Cloning Kit ClonExpress II One Step Cloning Kit ClonExpress Ultra One Step Cloning Kit . 5min Universal Ligation Mix (1 / 1) 1 BioLinks Lab Supplies. Nijenheim 1325. 3704VC Zeist. The Netherlands. Tel . Email [email protected]
· ClonExpress MultiS，bufferExnase MultiS。. ，。. ，Exnase MultiS、PCR。. PCRPCR
· ClonExpress MultiS One Step Cloning Kit 2259 451B
· .pdf,® ClonExpress MultiS One Step Cloning Kit C113 Vazyme biotech co., ltd. Version 7.1 Contents 01/02 02/ 02 03/ 02 04/ 02 05/ 02 06/ 03 07/ 03
， C113-01 ClonExpress® MultiS One Step Cloning Kit, C113-01 ClonExpress® MultiS One Step Cloning Kit，3 ，
ClonExpress II One Step Cloning Kit-——-ClonExpress®IIOneStepCloningKit 、、，50bp-10kbDNA
· The assembly of multiple DNA fragments in plasmid construction was performed by using the ClonExpress MultiS One Step Cloning Kit (Vazyme Biotech
· ClonExpress® MultiS One Step Cloning Kit ISO 9001 2015 SYSTEMS 01 02 Contents 02/ 01/ ClonExpress®、DNA，
ClonExpress MultiS One Step Cloning Kit. 63.00 €131.00 €. excl. VAT plus shipping. Size. Choose an option 10 RXNS 25 RXNS. Clear. ClonExpress MultiS One Step Cloning Kit quantity. Add to cart. Categories Cloning & Mutagenesis, Fast Cloning, Molecular Biology SKU C113.
· Phanta HS Master Mix (VP512-01) and ClonExpress MultiS One Step Cloning Kit (C113-02) from Vazyme Biotech. RFP-labeled Salmonella typhimurium was a gift from John Brumell (32). cDNAs for ZDHHC5, ZDHHC20, NOD2, and NOD1 were gifts from Zongping Xia. The NF-κB reporter pNiFty3-Luc (pnf3-lc2) was purchased from Invivogen. Latex beads 3.84 µm
· ClonExpress MultiS One Step Cloning Kit （）（C113） ￥520 TUNEL BrightGreen Apoptosis Detection Kit（）（A112） ￥1780
· ClonExpress MultiS One Step Cloning Kit (Vazyme Biotech Co., Ltd). 20 μL reaction mixture containing 4 μL 5×CE MultiS Buffer, 2 μL Exnase MultiS, ~50 ng linear pET28a plasmid (pre- digested with NdeI/XhoI), ~20 ng fragment 1 (~ 900 bp), ~10 ng fragment 2 (~ 280 bp) and
· ClonExpressMultiSOneStepCloningKitisanewversioncloningkitbasedonClonExpressOneStepCloningtechnology.ExnaseMultiSandreactionbufferinthiskitareespeciallyoptimizedfortheassemblyofmultiplefragments.Withthehelpof thiskit,up tofivefragmentscan be orderly assembled in a single reaction. In addition, Exnase MultiS is compatible with the reaction systems of
· .pdf,® ClonExpress MultiS One Step Cloning Kit C113 Vazyme biotech co., ltd. Version 7.1 Contents 01/02 02/ 02 03/ 02 04/ 02 05/ 02 06/ 03 07/ 03
· The SP(VmE02)-INF1 SP, SP(PR1)-GFP, and SP(VmE02)-GFP expression constructs were generated using ClonExpress MultiS One-Step Cloning Kit (Vazyme). For generation of VmE02 genecomplementation constructs, PVX- VmE02 vector was used as template to amplify the sequence, and the fragments were then ligated to pDL2- GFP vector (Zhou et al ., 2011
· 1 ClonExpress TM II 2 λDNA 500 bp ， ClonExpress TM II One Step Cloning Kit pUC 19 EcoRI HindIII 。 PCR 。M Marker III；1-24, 24 。
· ClonExpress MultiS One Step Cloning Kit （）（C113）ClonExpress® MultiS One Step Cloning Kit、、，
· ClonExpress MultiS One Step Cloning Kit （）（C113 ） （Vazyme） ￥ TUNEL BrightGreen Apoptosis Detection Kit（
· ClonExpress MultiS One Step Cloning kit is a new version cloning kit based on ClonExpress One Step Cloning technology. Exnase MultiS and reaction buffer supplied in this kit are especially optimized for multi-insertion seamless cloning (MultiS for short). With the help of this kit, sequential assembly of up to five insertions can be realized in
ClonExpress II One Step Cloning (Vazyme, #C112) is a simple, fast, and high efficient cloning technology which is based on a homologous recombination technology. It enables directional insertion of any amplified DNA product into any linearized vector at any site. Firstly, the vector is linearized at the cloning
· ClonExpress ® Ultra One Step Cloning Kit，1-5，2×ClonExpress Mix，50℃5-15 min，。. . C115-01 (25 rxn) C115-02 (50 rxn) 2 ×ClonExpress ® Mix. 125 μl. 2 ×125 μl. pCE
· ClonExpress® Ultra One Step Cloning Kit C115-02 2-3 -DeepBio 5567.2 DeepBio ，，，
ClonExpress MultiS One Step Cloning Kit (Vazyme, #C113) is a new version cloning kit based on ClonExpress One Step Cloning technology. Exnase MultiS and reaction buffer in this kit are especially optimized for the assembly of multiple fragments. With the help of this kit, up to 5 fragments can be orderly assembled in a single reaction.
· ClonExpress MultiS One Step Cloning Kit (Vazyme, Nanjing, China) to yield a CYP expression vector, pRS426-HXT7p-FBA1t. The ORF of CYP candidates was cloned into pRS426-HXT7p-FBA1t to yield a series of CYP gene carrying plasmids using primer pairs described in Table S1. Another helper plasmid pRS425-TEF1p-PGK1t was con-
· ClonExpress MultiS One Step Cloning Kit （）（C113）
ClonExpress MultiS One Step Cloning Kit C113-01/02 ClonExpress®MultiSOneStepCloningKit
· ClonExpress.pdf,ClonExpressTM II One Step Cloning Kit Vazyme Biotech Co., Ltd w w /Web w . v /Tel a Vazyme biotech co., ltd. z y /Sales [email protected] m e . c
· The PCR products were inserted into the digested pET28a plasmids (BamHI) using ClonExpress MultiS One Step Cloning Kit (Vazyme, China) generating the pET28a-transcriptional regulator plasmid. The successfully constructed plasmids were transformed into E. coli BL21 (DE3) strain respectively. Subsequently, a single colony on the plate was
· ClonExpress MultiS One Step Cloning kit is a new version cloning kit based on ClonExpress One Step Cloning technology. Exnase MultiS and reaction buffer supplied in this kit are especially optimized for multi-insertion seamless cloning (MultiS for short). With the help of this kit, sequential assembly of up to five insertions can be realized in
· ClonExpress MultiS One Step Cloning Kit （）（C113） ￥520 TUNEL BrightGreen Apoptosis Detection Kit（）（A112） ￥1780
· Recombination was conducted using the ClonExpress MultiS One Step Cloning Kit (Vazyme, Nanjing, China). Golden Gate assembly protocols were designed using j5 software tool (Revision as of
· 50 rxn. . ￥1000.0. ClonExpress®II One Step Cloning Kit. . 、、，. . 50 bp-10 kb DNA . PCR .
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