Phusion® U DNA polymerase is a novel engineered high fidelity enzyme developed using fusion technology.
· Phusion U Multiplex PCR Master Mix contains all the mplate DNA and primers. The master mix composition is designed to give optimal results in simultaneous amplification of multiple targets ranging in size from 70 to 2,500 bp. It contains a proprietary buffer and balanced ratio of all
Includes • Phusion U Hot Start DNA Polymerase, 2 U/µL • 5X Phusion Green HF Buffer • 5X Phusion Green GC Buffer • DMSO Both Phusion Green HF Buffer and Phusion Green GC Buffer provide 1.5 mM MgCl 2 in the final 1X concentration. Store at -20°C.
Thermo Scientific Phusion U DNA polymerase is a novel engineered high fidelity enzyme developed using fusion technology. Due to a proprietary mutation in the so called dUTP binding pocket of Phusion, Phusion U overcomes an important limitation of proofreading enzymesit is able to incorporate dUTP and read through uracil present in DNA templates.
· Phusion DNA (Phusion DNA Polymerase) Phusion DNA Polymerase,。Pfu DNADNASso7d,
Phusion U and Phusion U Green Multiplex PCR Master Mixes contain Phusion U Hot Start DNA Polymerase, 2X reaction buffer, 3 mM MgCl2, 400 °m of each dATP, dTTP, dCTP and dGTP. The master mixes are supplied with nuclease-free water.
Thermo Scientific Phusion U DNA 。 Phusion dUTP ，Phusion U dUTP DNA 。
· Thermo Scientific™ Phusion™ U Hot Start DNA Polymerase is a modified Phusion DNA Polymerase which efficiently amplifies uracil-containing templates. It can be used to minimize carry-over contamination in conjunction with dUTP and uracil-DNA glycosylase (UDG) and for amplification of bisulfite-treated and damaged DNA. A unique processivity-enhancing domain makes this Pyrococcus-like proofreading enzyme extremely processive, accurate and rapid. Phusion U Hot Start DNA Polymerase is capable of amplifying long amplicons such as the 7.5 kb genomic and 20 kb λ DNA used in Thermo Fisher Scientific quality control assays. Phusion U
· Description. Thermo Scientific Phusion U DNA polymerase is a novel engineered high fidelity enzyme developed using fusion technology. Due to a proprietary mutation in the so called dUTP binding pocket of Phusion, Phusion U overcomes an important limitation of proofreading enzymesit is able to incorporate dUTP and read through uracil present in DNA templates.
· Phusion U Multiplex PCR Master Mix Pub. No. MAN Rev. Date 27 June 2018 (Rev. B.00) #__ Lot __ Expiry Date __ Store at -20°C possible, the primer should terminate with a G or C 2 used with unpurified samples such as blood or serum Master Mix The Phusion U Multiplex PCR Master Mi Ordering information Component Multiplex 2X Phusion U
Price (£) M0530L 500 UNITS 2,000 units/ml £371.00 M0530S 100 UNITS 2,000 units/ml £81.00 For high speed and high performance PCR. Manufactured and quality-controlled at New England Biolabs, Thermo Scientific ® Phusion High-Fidelity DNA Polymerase offers both high fidelity and robust performance.. 50X higher fidelity than Taq. Robust reactionsmaximal success with minimal
Thermo Scientific Phusion U PCR Master Mix is a ready-to-use, 2X end-point PCR master mix designed for simultaneous amplification of multiple targets in a single tube. Over 20 primer pairs may be combined into a single reaction for highly specific and efficient multiplexing over a broad range of primer and template concentrations.
The Phusion U Multiplex PCR Master Mix contains a proprietary buffer with balanced concentrations of all PCR components eliminating the need for tedious optimization. The Phusion U Green Multiplex PCR Master Mix (F-564S or F-564L) further simplifies the workflowit includes a density reagent and two electrophoresis tracking dyes for direct loading of PCR products on gels.
· The genetically encoded pHusion sensor and a novel mounting technique presented in this study make it possible to record pH changes upon treatment, with minimized impact on the sensitive organ. pHusion is expressed well throughout the Arabidopsis plant body when expressed from the strong constitutive 35S promoter. This is optimal for expression
· Thermo Scientific Phusion U PCR Master Mix is a ready-to-use, 2X end-point PCR master mix designed for simultaneous amplification of multiple targets in a single tube. Over 20 primer pairs may be combined into a single reaction for highly specific and efficient multiplexing over a broad range of primer and template concentrations.
Phusion® U multiplex PCR master mix is a ready-to-use, 2X end-point PCR master mix designed for simultaneous amplification of multiple targets in a single tube.
Thermo Scientific Phusion U DNA Polymerase is a proofreading enzyme (25x Taq) with a proprietary mutation to read through uracils and incorporate dUTP
The Phusion U Hot Start DNA Polymerase is an enzyme that is combined with a reversibly bound, specific Affibody protein. Binding of the Affibody protein inhibits DNA polymerase and 3’-5’ exonuclease activities at ambient temperatures, which allows you easy pipetting. Moreover, usage of Phusion U Hot Start DNA Polymerase avoids nonspecific
· 5x Phusion HF Buffer 10μl 1x 10mM dNTPs 1μl 200μM each primer A x μl 0.5μM primer B x μl 0.5μM template DNA xμl H 2 O add to 50μl Phusion DNA polymerase (2U/μ) 0.5μl 0.02 U/pl A 2x supermix is now available containing either HF buffer or GC buffer, dNTPs, and Phusion polymerase. See references section below for links.
Phusion U Hot Start Green DNA Polymerase is a combination of Phusion U Hot Start DNA Polymerase and 5X Phusion Green Buffers. The buffers include a density reagent and two tracking dyes for direct loading of PCR products on a gel. The colored buffer does not interfere with Phusion U performance and is compatible with downstream applications
Thermo Scientific Phusion High-Fidelity DNA Polymerases set a gold standard for high performance PCR. Featuring an error rate 50-fold lower than that of Taq, and 6-fold lower than that of Pfu, Phusion High-Fidelity DNA Polymerase is excellent choice for cloning and other applications requiring high fidelity. Phusion DNA Polymerases offer robust performance with short protocol times, even in the presence
Includes Phusion U and Phusion U Green Multiplex PCR Master Mixes contain Phusion U Hot Start DNA Polymerase, 2X reaction buffer, 3 mM MgCl2, 400 °m of each dATP, dTTP, dCTP and dGTP. The master mixes are supplied with nuclease-free water.
Thermo Scientific Phusion U DNA polymerase is a novel engineered high fidelity enzyme developed using fusion technology. Due to a proprietary mutation in the so called dUTP binding pocket of Phusion, Phusion U overcomes an important limitation of proofreading enzymesit is able to incorporate dUTP and read through uracil present in DNA templates.
· Q5 DNA Polymerase concentration. Q5 High-Fidelity DNA Polymerase is recommended to be used at a final concentration of 20 U/mL (1.0 U/50 μl reaction). However, the optimal concentration of Q5 High-Fidelity DNA Polymerase may vary from 10–40 U/mL (0.5–2 U/50 μl reaction) depending on amplicon length and difficulty.
· Use Phusion DNA Polymerase at 0.5–1.0 U per 50 μL U/50 μL (see section 4.1). Use 200 μM of each dNTP. Do not use dUTP (see section 4.3). Phusion DNA Polymerases produce blunt end DNA products. 3. Guidelines for using Phusion DNA Polymerase Carefully mix and centrifuge all tubes before opening to ensure homogeneity and improve recovery.
· The genetically encoded pHusion sensor and a novel mounting technique presented in this study make it possible to record pH changes upon treatment, with minimized impact on the sensitive organ. pHusion is expressed well throughout the Arabidopsis plant body when expressed from the strong constitutive 35S promoter. This is optimal for expression
Phusion DNA Polymerase is an ideal choice for cloning and can be used for long or difficult amplicons. With an error rate > 50-fold lower than that of Taq DNA Polymerase and 6-fold lower than that of Pyrococcus furiosus DNA Polymerase (1), Phusion is one of the most accurate thermostable polymerases available. Phusion DNA Polymerase possesses 5´→ 3´ polymerase activity, 3´→ 5´
Includes Phusion U and Phusion U Green Multiplex PCR Master Mixes contain Phusion U Hot Start DNA Polymerase, 2X reaction buffer, 3 mM MgCl2, 400 °m of each dATP, dTTP, dCTP and dGTP. The master mixes are supplied with nuclease-free water.
· Phusion DNA，。PhusionPCR （），555125（20μl ），4.44，1878 500，！
Thermo Scientific Phusion U Green Multiplex PCR Master Mix is a ready-to-use, 2X end-point PCR master mix designed for simultaneous amplification of multiple targets in a single tube. Over 20 primer pairs may be combined into a single reaction for highly specific
· Product codes F-530S, 100 U F-530L, 500 U Stable for one year from the assay date. Store at -20°C. 1. Introduction Finnzymes’ Phusion™ High-Fidelity DNA Polymerase offers extreme performance for all PCR applications.
￥2800. thermo. Phusion High-Fidelity DNA Polymerase (2 U/μL) F530L F530L. . . . .
· The genetically encoded pHusion sensor and a novel mounting technique presented in this study make it possible to record pH changes upon treatment, with minimized impact on the sensitive organ. pHusion is expressed well throughout the Arabidopsis plant body when expressed from the strong constitutive 35S promoter. This is optimal for expression
Answer. Phusion Hot Start II DNA polymerase concentration is optimized to give good results in most reactions. When the PCR reaction is set up according to the instructions, the final concentration of Phusion enzyme is 1 U in 50 µL reaction (0.4 U in 20 µL reaction). View more ›.
· Phusion • 0.4 U / 20 µl rxn • 3 min extension time • 15 of 16 clones amplified Pyrococcus furiosus • 1.0 U / 20 µl rxn • 10 min extension time • 9 of 16 clones amplified Thermus aquaticus • 0.5 U / 20 µl rxn • 3 min extension time • 10 of 16 clones amplified Figure 5 (on the right).
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